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Microsoft PowerPoint, however, is presentation software. The only way to properly ensure the integrity of the resolution of your original image is to open it in Photoshop at the size and resolution at which it was acquired. Digital images may also be acquired using a scanner or a phospho- or fluorescence-imager, and it is also important to know the resolution at which these types of images were acquired.Ĭhoosing the correct application: The problem with PowerPointĪdobe Photoshop is an image-processing application. If the total number of pixels in the file you create is greater than the total number of pixels in your original, the computer has just created image data for you that was not present in the original. When that file is imported into an image manipulation application such as Adobe Photoshop, you may get an option to set the size and resolution of the image. You should be aware of the resolution at which the image was acquired by the digital camera on your microscope. Thus, a 1 inch × 1 inch image (too small for printing) at 900 dpi will convert into a 9 inch by 9 inch image at 100 dpi (insufficient resolution for printing) or a 3 inch × 3 inch image at 300 dpi (just right for printing). That is, you can resize an image without altering the number of pixels. Note that, with the right settings in Photoshop, physical size and resolution can be traded off against each other without a gain or loss in the amount of information. This can generate an image of ∼2,400 × 2,400 pixels, or 8 inches × 8 inches at 300 dpi. The best digital cameras these days can acquire an image of 6 megapixels in size. This has been determined by our printer as the minimum resolution required to prevent “pixelation” of images (i.e., to not see individual pixels) when the image is printed on paper.
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Note that this should be the resolution of the image at the dimensions at which you want to have it printed. The resolution requirements for the JCB are listed below (for more detailed information, see Instructions to Authors: ). We thus maintain the American-centric request that resolution be measured in dpi. The JCB is printed in the United States, and certain steps in the production process do not handle files measured in dots per centimeter (Adobe Solutions Network, 2003). Thus, 300 dpi indicates a resolution of 300 pixels per inch × 300 pixels per inch = 90,000 pixels per square inch. Although resolution is defined by area, it is often described using a linear measurement, dots per inch ( dpi). The size of a pixel can vary, and the resolution of an image is the number of pixels per unit area. A pixel is a square (or dot) of uniform color in an image. For the purposes of this editorial, image size is defined as the total number of pixels in the image. People refer to the size of an image in various ways, such as length by width, total number of pixels, or number of megabytes that it takes to store on a computer disk. This converter will be used to convert authors' RGB files to CMYK for printing. Our printer is working on an RGB to CMYK converter that will produce better print quality than the built in converter in Adobe Photoshop, because it will be specific to the profile of their color printing presses. As such, authors will now receive their figures proofs only electronically, and they must understand that the print version may not have the same color quality. This does not mean that we want the print version to look substandard, but we will not be using the print version as our standard. With a completely electronic work flow, we have now decided to make the online version of the journal the “journal of record” and to optimize the reproduction of color images online by posting them without any conversion of the colors from the original. Thus, authors' original data was being converted from RGB format to CMYK and back to RGB for posting online, with a loss of quality in each conversion.Since June 1st of this year, the JCB has encouraged all authors to submit their manuscripts electronically. These figures were then converted to RGB for posting online. In the mixed work flow of the transition to a completely electronic work flow (when we were ac-cepting both hard copies and electronic files), we requested that authors submit files in CMYK format to match the format in which hardcopy figures were scanned for printing. Color scans were done in CMYK format, to match the printing mode. Before we accepted electronic figure files from authors, all figures were scanned to produce files to incorporate into our layout software. In particular, we have taken pride in the quality of color image reproduction. The JCB has always worked to reproduce the highest quality images in print.